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mRNA Processing and Metabolism

mRNA Processing and Metabolism

Daniel R. Schoenberg
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A collection of readily reproducible techniques for the study of mRNA processing and metabolism. These cutting-edge techniques range from cotranscriptional processing events that occur while the mNA is engaged with elongating RNA polymerase II, to in vivo and in vitro splicing and its biochemical analysis, and alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. Each proven protocol is described in step-by-step detail and contains a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding know pitfalls.
年:
2004
版本:
1
出版商:
Humana Press
語言:
english
頁數:
267
ISBN 10:
1592597505
ISBN 13:
9781592597505
系列:
Methods in Molecular Biology
文件:
PDF, 4.89 MB
IPFS:
CID , CID Blake2b
english, 2004
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